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Work out MPN of B. cereus cells/g of sample (see BAM Appendix 2) based on the quantity of tubes at each dilution where the presence of B.
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Permit inoculum to get fully absorbed ahead of incubating for twenty-four h at 30°C. Verify plates for lecithinase manufacturing as indicated by zone of precipitation surrounding progress. Mannitol is not really fermented by isolate if advancement and bordering medium are eosin pink. (Yellow coloration implies that acid is developed from mannitol.) B. cereus colonies are frequently lecithinase-optimistic and mannitol-detrimental on MYP agar.
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